Isolation of Caffeine from a Tea Bag - 1788 Words

Experiment 11b: Isolation of Caffeine from a Tea Bag October 12,2012 Purpose: The objective of this experiment is to extract caffeine from tea bags using the method of extraction. After the caffeine is extracted it is then compared to the nutritional facts amount per serving on the tea bags box. Supplies: Glassware: 1. 50.0 m L graduated cylinder 2. 150.0 mL beaker 3. 2-glass stirring rod 4. 6-test tubes 5. 2-glass pipettes 6. 2-50.0 mL Erlenmeyer flasks 7. glass funnel Hardware: 1. hot plate (Corning PC-420P) 2. electronic scale (S/N: 8330280171) 3. stapler with at least 2 staples 4. test tube rack 5. hot plate tile 6. refrigerator 7. 7-rubber stoppers 8. centrifuge (Becton Dickinson; Clay Adams Brand†¦show more content†¦Place the Erlenmeyer flask and the methylene chloride on a hot plate that is located under a fume hood. 29. Set the hot plate to 200.0 oC. 30. Place a glass stirring rod into the Erlenmeyer flask to avoid it boiling over. 31. Allow the methylene chloride to evaporate completely. 32. Using a hot glove, remove the Erlenmeyer flask from the hot plate and place it on a hot plate tile so it can cool. The powder-like substance left in the Erlenmeyer flask is the caffeine. 33. Turn off the hot plate. 34. Once cool, weigh the mass of the Erlenmeyer flask using an electronic scale. Record its mass. 35. Record all observations. 36. Put a rubber stopper in the Erlenmeyer flask that contains the caffeine. 37. With a piece of masking tape and a pen, label the caffeine flask with the names of the lab group members. 38. Dispose of chemical waste in a halogenated waste container. 39. Clean up working area. Data/Observations: Mass of tea 4.670 g Mass of empty Erlenmeyer flask 40.559 g Mass of Erlenmeyer flask after evaporation 40.671 g Mass of extracted caffeine (40.671g-40.559g) 0.112 g Observations: the caffeine left in the bottom of the Erlenmeyer flask appears to be an off-white color, powder-like substance. Tetley claims there is between 40 to 50 milligrams of caffeine in a typical eight-ounce cup of brewed black tea or green tea steeped for three to five minutes (Tetley). Using this information itShow MoreRelatedIsolation of Caffeine from Tea Leaves1831 Words   |  8 PagesIsolation of Caffeine from Tea Leaves Introduction Caffeine is a member of the class of compounds organic chemists call alkaloids. Alkaloids are nitrogencontaining basic compounds that are found in plants. They usually taste bitter and often are physiologically active in humans. The names of some of these compounds are familiar to you even if the structures aren’t: nicotine, morphine, strychnine, and cocaine. 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